DENGUE VIRUS - SCI AND TECH

News: Prior infections driving dengue virus evolution in India

 

What's in the news?

       While the first infection with any of the four dengue serotypes can prevent reinfection by the same serotype for a long period.

       The second infection by a different serotype can have a very high viral load and cause severe disease.

       This is because the cross-protection offered by the first infection acts as a shield against other serotypes only for two-three years and then begins to drop.

       This is because while the antibodies are not able to neutralize the virus belonging to different serotypes, the virus is better able to bind to the antibodies leading to higher cell infection and thus enhanced severity and viral load.

       This is called the antibody-dependent enhancement mediated by cross-reactive antibodies.


Key takeaways:

       In a nutshell - “While the virus that is identical to the one that caused the first infection will be neutralized for a long time, viruses that are a bit look-alike of the serotype that caused the first infection have greater ability to take advantage of the weakened immune responses and bind to pre-existing antibodies and cause severe disease than the three other serotypes that did not cause the first infection”.

 

Dominant Serotype:

       The DENV-4-Id lineage is dominant in South India, and about 50% of infections in South India are due to this India-unique lineage.

 

Evolution through Antibody dependent enhancement:

       The E gene, which is seen across the dengue virus exterior, plays an important role in binding to the cell receptors.

       The substitution rate for the E gene was highest at 44% in genotype I of DENV-4 compared with the whole genome, suggesting high immunological pressure driving the divergence of the DENV-4 E gene.

       Most dengue virus evolution happens in the E gene.

       This suggests that evolution is driven primarily due to interaction with the host immune system.

 

Evolution of Dengue virus and vaccine efficiency:

       The evolution of the viruses across serotypes has implications for vaccine efficacy. The majority of strains used for developing dengue vaccines are based on strains isolated between 1964 and 1988.

       Indian variants of DENV-1 and DENV-4 are distinct from all the vaccines compared to DENV-2 and DENV-3.

 

Go back to Basics:

Dengue:

       Dengue is a mosquito-borne tropical disease caused by the dengue virus (Genus Flavivirus), transmitted by several species of female mosquito within the genus Aedes, principally Aedes aegypti.

       There are 4 distinct, but closely related, serotypes (separate groups within a species of microorganisms that all share a similar characteristic) of the virus that cause dengue (DEN-1, DEN-2, DEN-3 and DEN-4).

       India recorded 63,280 dengue cases as of September, 2022.

 

Symptoms: Sudden high fever, severe headaches, pain behind the eyes, severe bone, joint, and muscle pain, etc.

 

Diagnosis and Treatment: Diagnosis of dengue infection is done with a blood test. There is no specific medicine to treat dengue infection.

 

Controlling Dengue Using Bacteria:

       Recently researchers from the World Mosquito Program have used mosquitoes infected with Wolbachia bacteria to successfully control dengue in Indonesia.

 

Method:

       The scientists infected some mosquitoes with Wolbachia and then released them in the city where they bred with local mosquitoes, until nearly all mosquitoes in the area were carrying Wolbachia bacteria. This is called the Population Replacement Strategy.

       At the end of 27 months, the researchers found that the incidence of dengue was 77% lower in areas where Wolbachia-infected mosquitoes had been released, as compared to areas without such deployments.

 

Dengue Vaccine:

       The dengue vaccine CYD-TDV or Dengvaxia was approved by the US Food & Drug Administration in 2019, the first dengue vaccine to get the regulatory nod in the US.

       Dengvaxia is basically a live, attenuated dengue virus which has to be administered in people of ages 9 to 16 who have laboratory-confirmed previous dengue infection and who live in endemic areas.

       Vaccine manufacturer Indian Immunologicals Limited (IIL) is developing India's first Dengue vaccine and has received permission for a Phase-1 trial.

       The vaccine is being produced in collaboration with the National Institutes of Health in the US.