RNA-based Antiviral for Plants – SCIENCE & TECHNOLOGY

NEWS: Scientists have developed an RNA-based antiviral technology that significantly strengthens plant immunity against devastating viral infections, especially the cucumber mosaic virus (CMV).

WHAT’S IN THE NEWS?

Overview of RNA-Based Antiviral Approach

  • Innovation Origin: Developed by a research team at Martin Luther University Halle-Wittenberg, Germany, the RNA-based antiviral offers a next-generation defence strategy against plant viruses.
  • Mechanism of Action: This method involves engineering double-stranded RNA (dsRNA), which is rich in small interfering RNAs (siRNAs), to initiate a strong immune response in plants.
  • Immune Trigger: The dsRNA is recognized by the plant’s immune system and processed into siRNAs, which then target and degrade the viral RNA with high precision.
  • Spray-Based Application: The antiviral can be delivered as a foliar spray, offering a non-genetically modified (non-GMO) solution that does not alter plant DNA.

 

Advantages of the RNA-Based Antiviral

  • Precision Targeting: Engineered siRNAs are designed to match and silence the most vulnerable parts of the viral genome, ensuring high specificity and efficiency.
  • Stronger and Durable Defence: The method enhances siRNA concentration within the plant, providing robust and longer-lasting protection against various virus strains.
  • Non-GMO Solution: Since it is applied externally as a spray, it bypasses the concerns associated with genetically modifying crops.
  • Adaptability: The dsRNA can be rapidly redesigned to counter new or mutating virus strains, making it a versatile and future-proof approach.

 

Potential Agricultural Impact

  • Reduction in Crop Losses: Could significantly mitigate the economic burden caused by plant viruses, which currently result in over $30 billion in losses annually.
  • Improved Food Security: Enhances the resilience of crops to viral threats, ensuring more stable food supplies, especially critical in regions heavily dependent on agriculture.
  • Support for Sustainable Agriculture: Offers an eco-friendly solution that aligns with the principles of integrated pest management and sustainable farming practices.

 

Challenges and Future Prospects

  • RNA Stability: RNA molecules are prone to degradation under field conditions, which may limit their efficacy unless stabilized.
  • Scalability of Production: Producing large volumes of dsRNA at a feasible cost remains a challenge for commercial deployment.
  • Regulatory Hurdles: Approval from agricultural and environmental regulatory bodies is necessary before this technology can be widely adopted.
  • Farmer Readiness: Adequate training and awareness among farmers are essential for the successful implementation of this new technology.

 

About Cucumber Mosaic Virus (CMV)

  • Host Range: CMV infects over 1,200 plant species, including vegetables, fruits, and ornamental plants, causing significant economic losses.
  • Transmission: Mainly spread by aphids in a non-persistent manner; also transmittable through infected seeds and mechanical contact.
  • Symptoms: Causes mosaic patterns on leaves, stunted growth, distortion of plant parts, and significant yield reductions.
  • Management Difficulty: Its broad host range and multiple transmission modes make CMV particularly hard to control.

 

Viral Infections in Plants: General Background

  • Nature of Infection: Plant viruses enter cells and hijack their replication machinery, leading to cellular damage and disrupted plant function.
  • Common Plant Viruses: Include CMV, Tomato Yellow Leaf Curl Virus, Potato Virus Y, and Tobacco Mosaic Virus, among others.
  • Symptoms: Include mosaic leaf patterns, leaf curling, chlorosis, fruit malformation, and plant death in severe cases.

 

Economic and Agricultural Impact of Plant Viruses

  • Yield Reduction: Viral infections can lead to yield losses of 25–70%, posing a major risk to agricultural profitability.
  • Global Financial Loss: UN FAO estimates that pests and diseases destroy around 40% of crops annually, with viruses causing $30 billion of this loss.
  • Plant Health Decline: Infected plants suffer stunted growth and produce unmarketable or lower-quality produce.

 

Factors Contributing to Viral Spread

  • Rapid Viral Mutation: High mutation rates in viruses allow them to evade natural plant immunity and resist control measures.
  • Vector Proliferation: Sap-sucking insects like aphids are major carriers; nearly 90 aphid species can transmit CMV alone.
  • Climate Change Effects: Rising temperatures and shifting rainfall patterns expand the habitat of vectors, intensifying viral outbreaks.
  • Absence of Direct Treatments: Unlike fungal or bacterial infections, there are currently no direct antiviral treatments available for plants.

 

India’s Vulnerability to Plant Viral Infections

  • Severe Yield Losses: CMV alone results in 25–30% yield losses in bananas and up to 70% in crops like cucumber, pumpkin, and melon.
  • Wide Crop Diversity: India’s agricultural system includes many virus-susceptible crops, increasing its exposure to viral threats.
  • Favorable Conditions for Vectors: Warm temperatures and monsoon humidity create ideal breeding conditions for aphids and other vectors.

 

Way Forward

  • Promoting Technological Adoption: Farmers should be trained and incentivized to use RNA-based antivirals such as Spray-Induced Gene Silencing (SIGS).
  • Combining with Traditional Practices: Integrating RNA technology with methods like crop rotation, resistant varieties, and vector management enhances effectiveness.
  • Farmer Awareness Campaigns: Outreach programs are essential to inform farmers about viral threats and modern protective strategies.
  • Governmental Support: Policymakers must prioritize regulatory clearance, financial assistance for farmers, and support for field trials and public-private collaborations.

Source: https://www.thehindu.com/sci-tech/energy-and-environment/rna-based-antiviral-offers-strong-defence-against-cucumber-mosaic-virus/article69497221.ece